Supplementary Figure 2: Spectral sensitivity of BLINK2 transfected in HEK293T cells.

(a) Representative whole-cell current traces recorded in HEK293T cells expressing BLINK2 channel. Voltage steps from + 80 to –100 mV, in the dark, 3 min after exposing the cell to 630-nm red light, 530-nm green light, 500-nm green light, 455-nm blue light and after the addition of 5 mM BaCl₂ in the external solution. (b) Current–voltage relationship of measurement in (a) in the dark (filled black circles), in 630-nm light (filled red circles), in 530-nm light (filled green circles), in 500-nm light (filled light-green circles), in 455-nm light (filled blue circles) and after the addition of 5 mM BaCl₂ in the external solution (open blue circles). Light of all wavelengths was provided at 90 μW/mm² intensity. The effect of each wavelength was tested versus the effect of blue light (455 nm) in 3 independent experiments.