Supplementary Figure 8: Passive membrane properties and intrinsic excitabilities of control and indicator-expressing neurons.
From: Fast, in vivo voltage imaging using a red fluorescent indicator
(a) AP FWHM and amplitude in GFP and VARNAM-transfected primary neurons. P = 0.47 and P = 0.35, respectively, two-tailed Mann–Whitney U test; n = 4 neurons (control), 6 neurons (VARNAM). (b) FWHM and amplitudes of spike waveforms from neurons electroporated with indicated constructs and control cells in acute brain slices. P > 0.99, Kruskal–Wallis test with Dunn’s multiple comparisons correction; n = 4 neurons per condition (control, Ace-mNeon and VARNAM+CheRiff-EGFP), 7 neurons (VARNAM) and 8 neurons per condition (Ace-mRuby3 and VARNAM+Ace-mNeon). (c) Input resistance, (d) membrane capacitance and (e) holding current (VH = –65 mV) values of neurons electroporated with indicated constructs and control cells in acute slices. P > 0.99, Kruskal–Wallis test with Dunn’s multiple comparisons correction; n = 4 neurons per condition (control, Ace-mNeon and VARNAM+CheRiff-EGFP), 7 neurons (VARNAM) and 8 neurons per condition (Ace-mRuby3 and VARNAM+Ace-mNeon). (f) Mean ± s.e.m. spike rate during a 300-ms epoch plotted as a function of the amplitude of current injection for control, VARNAM and Ace-mNeon-electroporated neurons. (g) Rheobase values of control, VARNAM and Ace-mNeon-neurons in (f). P > 0.99, Kruskal–Wallis test with Dunn’s multiple comparisons correction; n = 7, 5 and 6 neurons for control, VARNAM and Ace-mNeon conditions, respectively. (a-e), (g), Values represent mean ± s.e.m.
