Supplementary Figure 2: Scarless 1-base substitution at TBX1 in the H9 hESC line. | Nature Methods

Supplementary Figure 2: Scarless 1-base substitution at TBX1 in the H9 hESC line.

From: Efficient scarless genome editing in human pluripotent stem cells

Supplementary Figure 2: Scarless 1-base substitution at TBX1 in the H9 hESC line.The alternative text for this image may have been generated using AI.

(a) Flow cytometric (FCM) analysis of transient (at day 2) and stable (at day 6) expression. (b) Before and after positive selection by magnetic-activated cell sorting (MACS) analyzed by FCM based on tCD19 expression. CD19-positive cells were enriched from 2.1% to 67.8%. (c) Fluorescent microscopy of single cell colonies after MACS positive selection. There were bright colonies (yellow arrowhead) and dim colonies (white arrowhead). Scale bar, 200 μm. (d) FCM analysis of mCherry in WT, dim, and bright clones. (e) Mean fluorescent intensity of mCherry analyzed by FCM. individual blue data points represent different clones (n = 3), and orange bars represent the mean value. (f) Schematic representation of primer design at TBX1 editing locus and PCR-based genotype analysis of single cell clones after MACS positive selection of first-round editing. FCM, fluorescent microscopy. PCR experiments were repeated three times, with similar results each time.

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