Supplementary Figure 6: Detection of random integration in RUNX1 and GFI1 editing after first-round editing.
From: Efficient scarless genome editing in human pluripotent stem cells
(a) Schematic representation of primer design for detection of random integration. Primer sets 1 and 2 can detect random integration if a selection marker is integrated into the genome with the sequence at the outside of the left and right homology arms, respectively (derived from plasmid backbone). PCR results are shown in (b) for RUNX1 editing and in (c) for GFI1 editing. PCR experiments were repeated twice, with the same result each time.
