Supplementary Figure 5: According to total cell extract RNA-seq, LncRNA XLOC_000304 is expressed at annotated coordinates,, but Proximity-CLIP reveals transcription initiation upstream of the annotated start site as divergent transcription from the CNN3 gene.
From: Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments
IGV tracks including APEX2-NES and H2B-APEX2 Proximity-CLIP reads, coverage and protein-bound clusters, as well as APEX2-NES and H2B-APEX2 Proximity-captured intact RNA and total cell RNA sequencing coverage. Data source, and coverage scales are depicted on the left side. Genomic coordinates and annotated Refseq genes are depicted on the top. Forward reads are colored red, reverse reads are in blue. Grey area zooms on the marked coordinates. We observed a similar expression pattern also for lncRNAs TUG1, ANRIL, NR_015404, CRNDE, AS_GARS and lincMKLN1_A1 divergently transcribed from MORC2, CDKN2A, MAPKAPK5, IRX5, GARS and MKLN1, respectively.
