Supplementary Figure 9: Control optical manipulations during volumetric functional imaging in Tg(elavl3:jRGECO1b) to test for direct effects of laser exposure, shutter noise, et cetera, on neural activity

Manipulated sites are indicated by yellow dots, targeting the DRN (a), IO (b) and nMLF (c), respectively. The weighted activity change is indicated by a green-magenta diverging color map. Weighting and scaling are identical to Fig. 6. Note that the maps are generally dimmer and strong peaks are absent in comparison to Fig. 6.The experiment shown in this figure was repeated independently once with similar results (please see SI Supplementary Fig. 10 for the second example).The experiment shown in this figure was repeated independently once with similar results (please see SI Supplementary Fig. 10 for the second example).Scale bar, 50 µm.