Supplementary Figure 1: Protocol for converting Drop-seq primer beads to DART-seq primer beads. | Nature Methods

Supplementary Figure 1: Protocol for converting Drop-seq primer beads to DART-seq primer beads.

From: Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells

Supplementary Figure 1: Protocol for converting Drop-seq primer beads to DART-seq primer beads.

Double-stranded toehold probes with a poly-(dA) ssDNA overhang are annealed to a subset of oligos on the surface of Drop-seq primer beads (left). The toehold is ligated to the bead with T4 DNA ligase. The complementary toehold strand is removed after ligation via heating. DART-seq beads (right) then contain custom primers as well as oligo(dT) tails from the original Drop-seq bead.

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