Supplementary Figure 2: A fluorescence hybridization assay characterizes the efficiency and tunability of the DART-seq ligation reaction.
From: Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
a) Assay design to test the efficiency of custom primer ligation to Drop-seq primer beads: (1) DART-seq beads are created withthe addition of custom primers at various concentrations, (2) oligos complementary to the custom primer, and labeled with Cy5, are hybridized to the DART-seq beads, and (3) the fluorescence signal of 3,000 beads is measured with a Qubit 3.0 fluorometer. b) Fluorescence signal as a function of the quantity of Cy5 oligos.
