Supplementary Figure 9: Cleavage in the presence of GuHCl or urea.
From: SNAC-tag for sequence-specific chemical protein cleavage
His-tag-T4L-GSHHW-3hbtmV2 (left) and His-tag-GSHHW-HB2225 (right). Cleavage conditions: 1 mM NiCl2, 0.1 M CHES, 0.1 M acetone oxime, 0.1 M NaCl, pH 8.2, protein 1 mg/mL, 22 °C, 18 h, GuHCl or urea concentrations are as indicated in the figure, 5 mM DPC for His-tag-T4L-GSHHW-3hbtmV2.
