Supplementary Figure 10: Peptide WCRLGSRHW cleavage.
From: SNAC-tag for sequence-specific chemical protein cleavage
Peptide 0.2 mM, 1 mM NiCl2, 0.1 M CHES, 0.1 M acetone oxime, pH 8.2, 22 °C. a. Cleavage reaction monitored at different time points. b. Mass measured by MALDI-TOF of uncleaved WCRLGSRHW-CONH2 (calc. 1,198.6 Da, obsd. 1,198.7 ± 0.2 Da) after 18 h, no obvious cysteine oxidation was observed. c. Mass measured by MALDI-TOF of cleaved peptide WCRL (calc. 633.3 Da, obsd. 633.3 ± 0.2 Da) after 18 h, no obvious cysteine oxidation was observed.
