Supplementary Figure 11: On-resin cleavage of His-tag-T4L-GSHHW-3hbtmV2 in different detergents. | Nature Methods

Supplementary Figure 11: On-resin cleavage of His-tag-T4L-GSHHW-3hbtmV2 in different detergents.

From: SNAC-tag for sequence-specific chemical protein cleavage

Supplementary Figure 11

Cleavage at pH 8.2, 0.1 M CHES, 0.1 M acetone oxime, 0.1 M NaCl, 1 mM NiCl2, 5 mM DPC, 22 °C for 18 h. Left, supernatant after on-resin cleavage; right, eluted protein remained on Ni-NTA beads after cleavage.

Back to article page