Supplementary Figure 12: Cleavage of optimized peptide sequences in short peptide constructs and in protein constructs.
From: SNAC-tag for sequence-specific chemical protein cleavage
Left: HPLC trace is the cleavage of peptides YFLPGSHHWG and YFLHNSHHWG. Cleavage conditions: 1 mM NiCl2, 0.1 M CHES, pH 8.2, 22 °C, peptide 0.2 mM. Middle: lane 2, t = 0; cleavage of His-tag-PGSHHW-HB2225 (lane 3) and His-tag-HNSHHW-HB2225 (lane 4). Cleavage conditions: 1 mM NiCl2, 0.1 M CHES, 0.1 M acetone oxime, 0.1 M NaCl, pH 8.2, protein 1 mg/mL, 22 °C, 18 h. Right: lane 2, t = 0; cleavage of His-tag-T4L-PGSHHW-3hbtmV2 (lane 3), His-tag-T4L-HNSHHW-3hbtmV2 (lane 4). Cleavage conditions: 1 mM NiCl2, 0.1 M CHES, 0.1 M acetone oxime, 0.1 M NaCl, pH 8.2, protein 1 mg/mL, 22 °C, 18 h, 5 mM DPC.
