Supplementary Figure 13: Cleavage time course for His-tag-T4L-GSHHW-3hbtmV2 (left) and His-tag-GSHHW-HB2225 (right).
From: SNAC-tag for sequence-specific chemical protein cleavage
Cleavage conditions: 1 mM NiCl2, 0.1 M CHES, 0.1 M acetone oxime, 0.1 M NaCl, pH 8.2, protein 1 mg/mL, 22 °C, 5 mM DPC for His-tag-T4L-GSHHW-3hbtmV2.
