Supplementary Figure 14: Positive control of TEV protease cleavage on His-tag-ENLYFQS-CPXR.
From: SNAC-tag for sequence-specific chemical protein cleavage
Cleavage conditions: 50 mM Tris, pH 8.0, 0.2 M NaCl, 1 mM DTT, 0.5 mM EDTA, 22 °C, 16 h, protein 2 mg/mL, TEV 0.02 mg/mL. Lane 1, before cleavage; lane 2, after cleavage.
