Supplementary Figure 15: Peptide YFLGSRHWGGSRRLFY (0.2 mM) cleavage at different pH, 1 mM NiCl2, 22 °C, 0.1 M CHES.
From: SNAC-tag for sequence-specific chemical protein cleavage
pH 8.2, t = 2 h (13% cleavage); 5 h (40% cleavage). pH 8.6, t = 2 h (34% cleavage); 5 h (78% cleavage). pH 9.0, t = 2 h (71% cleavage); 5 h (97% cleavage).
