Supplementary Figure 1: rNTase assay using RNase P-derived reporter RNA.

a, RNase P reporter RNA is co-expressed with MS2 coat protein-rNTase fusion in yeast. The tethered rNTase adds nucleotides to the 3ʹ end of the reporter RNA. b, Schematic of sample processing to detect adenosine tails or uridine tails added to the reporter RNA and example data identifying PUP or PAP activity. This tail-specific approach was also used to produce RT-PCR data in Fig. 1a. c, RT-PCR analysis to detect adenosine tails or uridine tails added to the RNase P reporter RNA by control rNTases, relative to empty vector or to no-reporter control. Lanes marked with a minus sign indicate reactions performed without reverse transcriptase. Representative gel image from 3 independent experiments.