Supplementary Figure 12: Investigation of the CLP clearance from plasma.
From: Bioluminescent-based imaging and quantification of glucose uptake in vivo
(a) Evolution of the whole-body BL-signal in time after injection of CLP in FVB-luc+/+ mice. The mice (n = 5) received i.v. injection of CLP (1.5 mM in 100 µl) and immediately after were imaged using CCD camera (IVIS Spectrum, PerkinElmer, USA). Images were acquired at 0, 4, 8, 12 and 24 hours post injection. Representative animal images are shown below. (b) Reduction of CLP concentration in blood plasma over time. Mice were injected with 1.5 mM CLP and the blood was collected at different time points. An excess of GAz4 was then added to blood plasma and the mixture was immediately added on top of 4T1-luc cells. The resulting BL signal was normalized to 1 µM luciferin signal in order to account for well to well cell number variation. Bars represent integrated area under the curve (total photon flux) over 30 minutes. Data are presented as mean ± SD (n = 5 in a, and n = 3 in b, biologically independent samples). (c) Evolution in time of whole-body BL-signal from FVB-luc+/+ mice treated with BiGluc probe. The mice received i.v. injection of 100 µL solution of CLP (1.5 mM in 0.1% BSA in PBS) followed by i.p. injection of GAz4 solution 24 h later. Bioluminescent signal acquisition started immediately after injection and was followed for 3 hours. Each point represents mean value (n = 3, biologically independent samples), error bars represent SD. (d) Signal to background ratio from various organs excised from FVB-luc+/+ mice 15 and 45 min after injection of BiGluc probe. Data are presented as mean ± SEM, n = 2 biologically independent samples.
