Supplementary Figure 14: Immunohistochemical staining of 18F-FDG PET/CT imaged 4T1-luc and 4T1-luc-GLUT1−/− no. 1 tumors and characterization of 18F-FDG positive 4T1-luc-GLUT1−/− no. 1 tumors.
From: Bioluminescent-based imaging and quantification of glucose uptake in vivo
(a) Tumors were stained with an antibody recognizing the GLUT1 protein. The blue and red squares indicate 4T1-luc and 4T1-luc-GLUT1-/- tumors that were stained, respectively. Scale bar = 50 µm. All experiments were performed independently four times. (b) Comparison of SUVmax values of 4T1-luc tumors and two 18F-FDG-positive 4T1-luc-GLUT1-/- no. 1 tumors that escaped CRISPR-mediated deletion of GLUT1. The pink arrow indicates 4T1-luc tumor and the green arrow indicates 4T1-luc-GLUT1-/- no. 1 tumor. P values were calculated using a two-tailed t-test, ns – p>0.05. The data are presented as the mean ± SEM (n = 10 in 4T1-luc tumors, and n = 2 in GLUT1-deficient tumors, biologically independent experiments). (c) Immunohistochemical staining of (b). Scale bar = 50 µM.
