Supplementary Figure 5: Native vs. Denatured MS of AquaporinZ (AqpZ) from E. coli.
From: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
Native spectrum was acquired on a Waters Synapt G1 Q-TOF with nanoESI via direct infusion while denatured spectra was acquired on a Waters Synapt G1 Q-TOF via nanoESI-LC-MS. The native sample (a.) and the denatured sample (b.) was acquired at a concentration of 10 µM. *Denotes the most abundant charge state. 24268.7 Da is the deconvoluted mass of the unmodified AqpZ monomer. Formylated AqpZ was also detected with a mass of 24,296.4 Da. ‡Five native tetramer masses were observed corresponding to five unique combinations of formylated and unformylated monomers.85
