Supplementary Figure 7: LC-MS of protein standard mixture run on Waters Acquity-Xevo G2-S QTOF.
From: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
Samples were prepared following the given SOP and separated using a PLRP-S (top panel of a, b, and c) or a C4 (bottom panel of a, b, and c) stationary phase. (a.) The final concentrations of each protein loaded onto the column were; 14 pmol ubiquitin, 49 pmol trypsinogen, 109 pmol myoglobin, and 64 pmol carbonic anhydrase. (b.) The final concentrations of each protein loaded onto the column were; 1.4 pmol ubiquitin, 4.9 pmol trypsinogen, 10.9 pmol myoglobin, and 6.4 pmol carbonic anhydrase. (c.) The final concentrations of each protein loaded onto the column were; 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin, and 0.64 pmol carbonic anhydrase.
