Fig. 5: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. | Nature Methods

Fig. 5: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase.

From: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

Fig. 5: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase.

Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

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