Fig. 6: LC-MS of bacteriorhodopsin-containing purple membrane of H. salinarum prepared following Protocol 5b and analyzed on an Agilent HPLC system coupled to a Thermo linear ion trap (LTQ) mass spectrometer.
From: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
Proteins were separated using an Agilent PLRP-S 300 Å, 2.1 × 150 mm, 3 µm. Supplementary Fig. 12 demonstrates this analysis on four additional instrumentation platforms.
