Supplementary Figure 3: Antibody Buffer and Gentle Elution Buffer Ablate MS Signal; MWCO-Ultrafiltration and Precipitation Rescue Signal.
From: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
Buffers included (a.) Thermo Gentle Elution Buffer (containing molar salt concentration), and (b.) Antibody buffer (10 mM Arginine, 10 mM Tris HCl, 10 mM histidine, 10 mM potassium phosphate, 10 mM citric acid, pH 5.5). All the above spectra were obtained using a Bruker SolariX FT-ICR mass spectrometer, 9.4T.
