Supplementary Figure 5: Heatmaps depicting editing efficiency for the on- and off- target sites.
From: Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9
Editing efficiency was measured by the log-transformed enrichment ratio (log2(E)) determined for each SpCas9 combination mutant. Enriched and depleted mutants have >0 and <0, respectively. To aid visualization, amino acid residues that are predicted to make contacts with the target DNA strand or located at the linker region connecting SpCas9’s HNH and RuvC domains are grouped on the y-axis, while those predicted to make contacts with the non-target DNA strand are presented on the x-axis. The combinations for those with no enrichment are indicated in gray.
