Supplementary Figure 6: Frequency of N20-NGG and G-N19-NGG sites in the reference human genome.
From: Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9
A custom Python code was used to find the occurrence of N20-NGG and G-N19-NGG sites in both strands of the reference human genome hg19, as an estimate of the targeting ranges of Opti-SpCas9 and other engineered SpCas9 variants including eSpCas9(1.1), SpCas9-HF1, HypaCas9, and evoCas9, respectively. N20-NGG sites are about 4.3 times more frequent than G-N19-NGG sites in the human genome.
