Supplementary Figure 8: Expression of SpCas9 variants in OVCAR8-ADR cells.
From: Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9
Cells were infected with lentiviruses encoding WT SpCas9, Opti-SpCas9, eSpCas9(1.1), HypaCas9, SpCas9-HF1, Sniper-Cas9, evoCas9, xCas9, or OptiHF-SpCas9. Protein lysates were extracted for Western blot analysis, and immunoblotted with anti-SpCas9 antibodies. Beta-actin was used as loading control. We failed to detect the expression of SpCas9-HF1 and xCas9 in OVCAR8-ADR cells, which could be due to their non-optimized sequence for expression in mammalian cells (ref. 24 and Nat. Biotechnol. 36, 888–893, 2018) and thus SpCas9-HF1 and xCas9 were not included in other activity assays. These experiments were repeated independently for three times with similar results.
