Supplementary Figure 11: Characterization of SpCas9 variants for editing target sites harboring sequences that are perfectly matched with the gRNA’s spacer or contain mismatch(es) using GFP disruption assay.
From: Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9
OVCAR8-ADR cells expressing WT SpCas9, Opti-SpCas9, eSpCas9(1.1), or HypaCas9 were infected with lentiviruses encoding gRNAs carrying no or one- to four-base mismatch(es) against the target. Editing efficiency was measured by cell percentage with depleted GFP level using flow cytometry. Values and error bars reflect the mean and s.d. of three independent biological replicates.
