Supplementary Figure 12: On-target editing activity of SpCas9 variants using truncated gRNAs.
From: Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9
a,b, OVCAR8-ADR cells expressing WT SpCas9, Opti-SpCas9, eSpCas9(1.1), or HypaCas9 were infected with lentiviruses encoding gRNAs of varied length (17 to 19 nucleotides) targeting the GFP sequence (a) and endogenous loci (b). Editing efficiency was measured by cell percentage with depleted GFP level using flow cytometry (a) and T7E1 assay (b). The list of gRNA sequences used is presented in Supplementary Table 5. For (a), values and error bars reflect the mean and s.d. of four independent biological replicates.
