Supplementary Figure 3: FANS by DNA content excludes nuclei multiplets.
From: Simultaneous profiling of 3D genome structure and DNA methylation in single human cells
(a) Single-nuclei FANS following standard in situ 3C procedure using a mixture of mESC and GM12878 results in a high fraction of wells containing both mouse and human nuclei. (b) Separate crosslinking of mESC and GM12878 nuclei followed by pooling and FANS eliminated wells containing both mouse and human nuclei. (c) Crosslinking under diluted condition reduced nuclei multiplets. (d) FANS selecting for 2N genomic content. (e) FANS selecting for 2N genomic content excluded the vast majority of nuclei multiplets.
