Supplementary Figure 6: Schematics and quality control of m1A-IP-seq and m1A-quant-seq libraries.
From: Evolution of a reverse transcriptase to map N1-methyladenosine in human messenger RNA
a, Schematic of library-construction procedures with evolved RT-130, and the final sequence composition of the library. b, Representative bioanalyzer analyses of NGS libraries built with RT-1306 for one of the three biological replicates. The library size is ~240 bp for all libraries with and without AlkB treatment. Considering the adaptor dimer is 132 bp, the average size of the fragmented insert is ~100 nt. c, Reproducibility examination based on correlation plots of transcriptome-wide coverage between replicates in both m1A-IP-seq and m1A-quant-seq. Each dot represents the coverages of one RefSeq-annotated RNA transcript (n = 38882) counted with ‘bedtools multicov’. At the sequencing data coverage depth of the current experiments (~100 million reads per library), transcriptome-wide coverages show decent reproducibility between replicates evaluated with Pearson’s correlation coefficient (r).
