Supplementary Figure 7: Location of mutation signatures within aligned reads.
From: Evolution of a reverse transcriptase to map N1-methyladenosine in human messenger RNA
a, IGV coverage and alignment tracks of three mRNA and lncRNA m1A sites. The alignment tracks show that mutations (labeled with colors, red for T and blue for C) are located distal to the ends of reads. b, Histograms of distributions of the mutation locations within the reads for all m1A sites (n = 614) called from m1A-IP-seq with P < 0.1 based on beta-binomial regression test in each library replicate. The normalized location of each mutation site is analyzed by the ‘bam-readcount’ program, in which 0 represents mutations come from either end of the read, and 1 represents the center of the read. Histograms show that distributions of read locations of mutations for m1A sites are mostly centered in the middle, rather than ends of aligned reads with mean ± s.d. normalized locations of 614 m1A sites in each library replicate.
