Supplementary Figure 11: Comparisons of mutation signatures captured by targeted library and m1A-quant-seq.
From: Evolution of a reverse transcriptase to map N1-methyladenosine in human messenger RNA
a, Number of sites identified by m1A-quant-seq, with P < 0.05 based on the beta binomial statistical test. b, Mutations captured by the targeted library for PRUNE and MALAT1 m1A sites are shown in IGV coverage traces, with and without AlkB treatment. c, Comparisons of mutation rates and AlkB sensitivities for the PRUNE and MALAT1 sites detected by targeted library and m1A-quant-seq. Error bars represent the s.d. of n = 3 technical targeted library replicates for targeted libraries and n = 3 cell culture replicates for m1A-quant-seq overlaid, with individual data points shown by dots.
