Supplementary Figure 3: Characteristics of DART-Seq datasets.

(a) Metagene analysis showing a density plot of the absolute distance of C to U editing sites from the stop codon (position 0). APOBEC1-YTH expression leads to C to U editing enriched in the vicinity of the stop codon, which mirrors the distribution of m⁶A. In contrast, APOBEC1-YTH^mut expression leads to editing throughout the 3’UTR (n = 3 independent samples per condition). (b) Pie chart showing the distribution of C to U editing sites in cells expressing APOBEC1 alone, which is characterized by a greater amount of intronic and intergenic editing events compared to APOBEC1-YTH-expressing cells (n = 2 independent samples). (c) Venn diagram showing overlapping C to U editing sites in DART-Seq libraries prepared from cells expressing APOBEC1-YTH^mut or APOBEC1 alone. (d) Distribution of exon lengths for internal exons containing C to U editing sites in APOBEC1-YTH-expressing cells. Exon lengths of internal exons from the human genome (hg19 annotation) are shown for comparison. There is a greater proportion of long internal exons among C to U edited exons in APOBEC1-YTH-expressing cells compared to the natural distribution throughout the transcriptome.