Supplementary Figure 3: A SCAPE 2.0, HiCAM volume acquired in a freely moving young adult C. elegans at 10 VPS.
From: Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0
The high pixel bandwidth of the HiCAM camera enabled dense sampling over a large 525x465x60 µm of view at 10 VPS. Image shows a single volume from a time-series on a freely moving C. elegans worm expressing panneuronal GFP (IM324: edls20[F25B3.3::GFP+rol-6(su1006)]). The colors represent depth into the sample and the data set has been square-rooted for visualization purposes to enhance contrast between the bright cell bodies and the dimmer dendritic process. See Supplementary Video 5 for full run, see Online Methods for experimental details, configuration AH. Scale bar 100 µm.
