Supplementary Fig. 8: Observed and calculated difference electron densities (DED) near the pCA chromophore. | Nature Methods

Supplementary Fig. 8: Observed and calculated difference electron densities (DED) near the pCA chromophore.

From: Time-resolved serial femtosecond crystallography at the European XFEL

Supplementary Fig. 8: Observed and calculated difference electron densities (DED) near the pCA chromophore.The alternative text for this image may have been generated using AI.

Left panels: observed difference electron density (blue: 3 σ, red: -3 σ contour levels). Right panels: calculated difference electron density (blue: 4 σ, red: -4 σ contour levels). Yellow model: structure of the dark (reference) state; blue model: structure at a particular time delay. a, 10 ps; b, 30 ps, c, 80 ps. In panel b pairwise difference density features are marked with α (negative) and β (positive). The feature γ shows the signal caused by the Cys-69 sulfur. The marked DED features can be readily detected at the other time delays. 13,142, 13,014 and 12,889 difference amplitudes were used to calculate the observed DED maps for a, b and c, respectively.

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