Extended Data Fig. 9: CRISPR-Cas3 editing in Klebsiella pneumoniae.
From: A compact Cascade–Cas3 system for targeted genome engineering
a, Growth curves of K. pneumoniae strains expressing distinct crRNAs targeting rfaH and sacX (2 each). Non-targeting crRNA expressing control is marked in blue. Values depicted are averages of 8 biological replicates each. b, Representative gel electrophoresis of PCR fragments amplified from 8 total surviving colonies each from the 4 crRNA targeting constructs (representing 1 biological replicate of 3 total). Primer pairs amplified regions flanking the targeted position at rfaH and sacX. Wild-type KPPR1 (wt) colonies were used as controls, L represents 1 kb DNA marker ladder. c, Percentage of survivors with targeted deletions at the targeted genomic positions. Values are averages of three biological replicates where 8 individual colonies were analyzed using site-specific PCR for each, error bars show standard deviations. d, Colony morphologies of deletion candidate strains of rfaH and sacX compared to wild-type K. pneumoniae KPPR1.
