Extended Data Fig. 5: BAcTrace expression in subsets of KCs induces labelling in PNs.

a, Negative control missing a split Gal4 hemidriver. Donor KCs: b, all, c, α’/β’, d, γ and e, α/β. f, Higher magnification view of the MB calyx and LH of a brain with Donor MB607B neurons (same as in D). The brain was mounted dorsal side up to provide better image quality of the MB calyx and LH. Within each lobe subtypes are: γ lobe: main (m) and dorsal (d), α’/β’ lobe: anterior-posterior (ap) and middle (m) and α/β: posterior (p), core (c) and surface (s). Images in A-F are representative of stainings from at least 3 animals. Maximum intensity projections of registered confocal stacks from age matched animals; (A), (B), (C) and (D) taken using the same microscope settings. Epitopes detected by antibodies are indicated in bold in each panel. Number of KCs per subtype from²⁴. Genotypes for each panel are described in Supplementary Table 16. Scale bars 30 µm.