Extended Data Fig. 9: BAcTrace reveals connections between PNs and LHNs.
From: BAcTrace, a tool for retrograde tracing of neuronal circuits in Drosophila
a, Split Gal4 lines LH173 and LH1139 drive GFP expression in cell types PV5c1 and AV6a1, respectively. Images for a representative brain of 5 animals for each line. AL: Antennal Lobe. b, Single slices from a representative AL and corresponding LH showing PNs labelled by expression of toxin in PV5c1 and AV6a1. Images are representative of PV5c1 (9 animals) and AV6a1 (7 animals). c, 3D renderings summarizing BAcTrace results for PV5c1 and AV6a1. d, Forward and reciprocal synapses between PNs for the 15 glomeruli analyzed in Fig. 5 and PD2a1/b1 (top) and AV1a1 (bottom) as assessed by EM. The number next to the glomerular identity of the PN is its EM identification number. Epitopes detected by antibodies are indicated in bold in (A) and (B). Genotypes for each panel are described in Supplementary Table 16. Scale bars (A) 50 µm and (B) 10 µm.
