Extended Data Fig. 3: Expression strength of VT033006-LexA::P65.
From: BAcTrace, a tool for retrograde tracing of neuronal circuits in Drosophila
a, V5 tag immunostaining of QF2::V5::hSNAP25::Syx in VK000018. b, GFP immunostaining of Syb::GFP in VK000037. Images in (A) and (B) are representative of stainings from 3 animals. c, Boxplot showing the fluorescence quantification per glomerulus for (A) and (B); each glomerulus was measured in 6 ALs from 3 brains. Leftmost panel in (A) and (B) are maximum intensity projections while all other panels are single slices from confocal microscopy stacks. Epitopes detected by antibodies are indicated in bold in (A) and (B). Scale bars 30 µm. Boxplot shows the median of the measurements. Lower and upper hinges correspond to the first and third quartiles; the upper whisker extends from the hinge to the largest value no further than 1.5. * inter-quartile range from the hinge. The lower whisker extends from the hinge to the smallest value at most 1.5 * inter-quartile range of the hinge.
