Extended Data Fig. 4: TEVT173V modestly increases BAcTrace efficiency.
From: BAcTrace, a tool for retrograde tracing of neuronal circuits in Drosophila
a) BAcTrace labelling of PNs from sparse KC Donors driven by MB005C using TEV variants. The panels for each condition are representative of stainings from 3 animals. (b) Constructs used in (C). (c) Targeting TEV to the synapse using the toxin receptor. The panels for each condition are representative of stainings from 4 animals. Maximum intensity projections of registered confocal stacks from age matched animals; images taken using the same microscope settings. Epitopes detected by antibodies are indicated in bold in (A) and (C). Genotypes for each panel are described in Supplementary Table 16. Scale bars 30 µm.
