Extended Data Fig. 4: enDUB-U21 has greater efficacy than enDUB-O1 in surface rescue of N1303K CFTR mutant channels.
From: Targeted deubiquitination rescues distinct trafficking-deficient ion channelopathies
a, Cumulative distribution histograms of Alexa647 fluorescence from flow cytometry analyses for cells expressing WT BBS-CFTR-YFP + nano (dotted line) and N1303K mutation co-expressing nano alone (red line), enDUB-O1 (cyan line), enDUB-U21 (blue line). Plot generated from population of YFP- and CFP-positive cells (n ≥ 5000 cells per experiment; N = 2). b, Same experimental design as above but with 24 hour incubation of VX809 with nano (green line), enDUB-O1 (cyan line) and enDUB-U21 (blue line) (N = 2).
