Extended Data Fig. 8: enDUB-U21CF.E3h deubiquitinates WT CFTR and N1303K.
From: Targeted deubiquitination rescues distinct trafficking-deficient ion channelopathies
a, Anti-ubiquitin labeling of WT CFTR and N1303K pulldowns. Lane 1 – untransfected cells; lane 2 – mCherry-CFTR + nanobody; lane 3 – mCherry-CFTR + enDUB-U21CF.E3h; lane 4 – mCherry-N1303K + nanobody; lane 5 – mCherry-N1303K + enDUB-U21CF.E3h. Proteins were pulled down with anti-mCherry and probed with anti-ubiquitin antibody (n = 2 independent experiments). b, Anti-CFTR labeling of WT CFTR and N1303K pulldowns. The top blot was stripped and probed with anti-CFTR antibody.
