Extended Data Fig. 2: Flow cytometry gating strategy for BTX-647 surface labeling experiments. | Nature Methods

Extended Data Fig. 2: Flow cytometry gating strategy for BTX-647 surface labeling experiments.

From: Targeted deubiquitination rescues distinct trafficking-deficient ion channelopathies

Extended Data Fig. 2: Flow cytometry gating strategy for BTX-647 surface labeling experiments.

a, Flow cytometry pseudocolor dot plots displaying an initial selection gate (left) for cells (vs debris); and a second selection gate (right) for singlets (vs doublets). b, Single color fluorescent controls after applying the gating strategy in a. c, Analysis gate established with single color controls in b to quantify channel surface density (BTX-647) in YFP- and CFP-positive cells (left) with cumulative distribution histograms (right). Sample 1 is exemplified by BBS-Q1-YFP + CFP-P2a-nano. Sample 2 is exemplified by BBS-Q1-YFP + CFP-P2a-nano + NEDD4L.

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