Extended Data Fig. 5: Robustness and reproducibility of DI-SPA.
From: Quantitative shotgun proteome analysis by direct infusion
Tryptic peptides from the MCF7 proteome (1 mg/mL) were analyzed 100 times with a shortened version of the parameter scouting method (Extended Data Fig. 3). a, TIC traces of the infusion data from injection #1, #25, #50, #75, #100, and those five overlaid. b, The number of peptide identifications from MSPLIT-DIA per analysis (FDR < 0.01) and (c) the distribution of peptide identifications summarized as a boxplot. The boxplot shows the median (percentile 50%) with an orange line, and the box represents the inner quartile range (IQR) Q1 and Q3 (percentiles 25 and 75). Whiskers show Q1 - 1.5*IQR and Q3 + 1.5*IQR.
