Supplementary Figure 4: Maxpar versus TOBis Organoid Barcoding in situ.
From: Cell-type-specific signaling networks in heterocellular organoids
a) De-barcoded cell counts from small intestinal organoids barcoded using the Maxpar Cell-IDTM Barcoding Kit either after removal from Matrigel (ex situ) or while still in Matrigel (in situ) (n = 2 independent samples). b) Mean dual counts of 110Pd from Matrigel incubated in PBS, Maxpar Cell-IDTM barcode #20 (106Pd, 108Pd, and 110Pd) resuspended in PBS (PBS + BC), or Maxpar Cell-IDTM barcode #20 resuspended in Maxpar Cell Staining Buffer (CSB + BC) (two-tailed unpaired t-test, p < 0.0001, n = 3 independent samples). Error bars represent standard deviation (SD). c) Maximum-gain confocal IF images of NHS ester-/C2 maleimide-Alexa 647 fluorescent probes shown in Fig. 3a to highlight background Matrigel staining. Each IF image is representative of three independent experiments. d) Small intestinal organoids concurrently stained in situ with Maxpar Cell-IDTM (102Pd, 104Pd, 105Pd, 106Pd, 108Pd, and 110Pd) and TOBis (124Te, 126Te, 128Te, 130Te, 196Pt, and 198Pt) barcodes using analogous 20-plex 6-choose-3 matrices. Data is representative of three independent experiments.
