Supplementary Figure 5: Maxpar versus TOBis Cell-Recovery Comparison.
From: Cell-type-specific signaling networks in heterocellular organoids
a) Example 10-plex barcoding workflow for organoid MC using Maxpar Cell-IDTM Barcoding Kit. b) Example 10-plex barcoding workflow for organoid MC using TOBis. c) Dissociation and d) centrifugation steps (s) required for multiplexed organoid experiments using Maxpar or TOBis barcoding. e) Single-cell recovery of serially-titrated small intestinal organoids barcoded with either Maxpar (ex situ) or TOBis (in situ) (two-tailed ratio-paired t-test, p < 0.001, n = 2 independent set of serially-titrated samples). f) Cell-type recovery (cell count) from equally seeded small intestinal organoids analyzed by MC and de-barcoded by Maxpar or TOBis. g) Cell-type recovery (population percentage) from equally sampled small intestinal organoid cells analyzed by MC and de-barcoded by Maxpar or TOBis (linear regression of correlation, R2 = 0.86, n = 2 independent samples). h) Mean debarcoded cell counts in the absence or presence of glutathione (GSH) in PBS washes following TOBis barcoding (two-tailed unpaired t-test, p < 0.0001, n = 3 independent samples). Error bars represent SD..
