Supplementary Figure 1: Design and characterization of a two-photon mesoscope equipped with a Bessel module. | Nature Methods

Supplementary Figure 1: Design and characterization of a two-photon mesoscope equipped with a Bessel module.

From: Rapid mesoscale volumetric imaging of neural activity with synaptic resolution

Supplementary Figure 1

(a) Schematics of the mesoscope. BE: beam expander, BR: beam reducer, MTS: motorized translation stage to switch between Gaussian (yellow path) and Bessel (red path) modalities, PBS: polarizing beam splitter, QWP: quarter waveplate, RFO: remote focus objective, VC: voice coil, Res.: 12kHz resonant scanner, PR: pupil relay, DM: dichroic mirror, and IO: imaging objective. (b–e) Lateral and axial point spread functions of 0.6-NA Gaussian and 0.4-NA Bessel foci measured at different field positions with n = 9 0.2-µm-diameter beads. Error bar: s.e.m.; FWHM: full width at half maximum. (f, g) CAD model of the Bessel mesoscope and the Bessel beam module, respectively. See Supplementary Data for a complete part list and an Autodesk Inventor® model of the Bessel module.

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