Supplementary Figure 3: Detailed PSF measurements across the entire imaging field of view.
From: Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
FWHMs of the lateral and axial PSFs of 0.6-NA Gaussian and 0.4-NA Bessel foci measured at different positions along (a-e) vertical, (f-j) horizontal, (k-o) and (p-t) diagonal directions, respectively, across the 5×5 mm2 field of view with 0.2-µm-diameter beads. N = 9 beads, error bar: s.e.m. The linearly polarized excitation light had its electric field along the y axis, leading to higher lateral resolution along the x axis. Data in Supplementary Fig. 1c are lateral FWHMs along horizontal direction with the excitation light polarized along x axis.
