Supplementary Figure 6: Detailed analysis of dendritic signals.

Same dataset as in Supplementary Fig. 5. (a) ΔF/F map, averaged across 12 consecutive frames (3.3 sec of image requisition). (b) Enlarged view of ΔF/F map in (a), with the ROIs for D1, D2 and D3 (used to calculate ΔF/F traces in Fig. 2g) shown in white outline. Magenta ROIs enclose dendritic spines. (c) Overlay of the ROIs on images of D1, D2 and D3 obtained with Bessel focus scanning. (d) Maximum intensity projection of Gaussian image stacks of the dendrites with structures color-coded by depth. Dendrite D1 spanned a depth range of z= 22 to 42 µm, D2 z= 8 to 80 µm, and D3 20 to 66 µm, respectively. (e, f) Fluorescence traces of segmented dendrites (D1, D2 and D3, black curves), spines (magenta curves), and dendritic shafts (green curves). The dendritic shaft signals were calculated by subtracting the magenta curves from the black curves.