Supplementary Figure 5: AAV-PHP.V2 Variant Exhibits Biased Transduction Towards Brain Vascular Cells.
From: Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types
a, Transduction of mouse brain by the AAV-PHP.V2 variant and control AAV9, carrying the ssAAV:CAG-mNeonGreen (n = 3, 3x1011 vg IV dose per C57BL/6J adult mouse, 3 weeks of expression) is shown. The sagittal brain images (left) are imaged under the same settings (also matched to the settings of sagittal brain images in Fig. 3c). Higher-magnification images of AAV-PHP.V2 transduced brain sections stained with αGLUT or αS100 or αOlig2 (magenta) are shown. b, Transduction of brain vasculature by AAV-PHP.V2 carrying ssAAV:CAG-DIO-EYFP (green) in Tek-Cre adult mice (left, 1x1012 vg IV dose per mouse, 4 weeks of expression) is shown, and its efficiency (right) is determined by the overlap of αGLUT1 staining (red) with EYFP expression across different brain areas (n = 2, mean of 3 images per brain region per mouse) c, Transduction of astrocytes by AAV-PHP.V2 in GFAP-Cre adult mouse (1x1012 vg IV dose per mouse, 4 weeks of expression) is shown. Percentage of cortical S100+ cells that overlapped with EYFP expression is quantified (n = 2, mean of 3 images per mouse).
